NAPPA Methods and Technology Development References

Advances in cell-free protein array methods. Yu X, Petritis B, Duan H, Xu D, LaBaer J. Expert Rev Proteomics. 2018 Jan;15(1):1-11. doi: 10.1080/14789450.2018.1415146. Epub 2017 Dec 13.

Automatic Identification and Quantification of Extra-Well Fluorescence in Microarray Images. Rivera R, Wang J, Yu X, Demirkan G, Hopper M, Bian X, Tahsin T, Magee DM, Qiu J, LaBaer J, Wallstrom G. J Proteome Res. 2017 Nov 3;16(11):3969-3977. doi:10.1021/acs.jproteome.7b00267. Epub 2017 Oct 10. 

Multiplexed Nucleic Acid Programmable Protein Arrays. Yu X, Song L, Petritis B, Bian X, Wang H, Viloria J, Park J, Bui H, Li H, Wang J, Liu L, Yang L, Duan H, McMurray DN, Achkar JM, Magee M, Qiu J, LaBaer J. Theranostics. 2017 Sep 20;7(16):4057-4070. doi: 10.7150/thno.20151. eCollection 2017. 

Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays. Tang Y, Qiu J, Machner M, LaBaer J. Curr Protoc Cell Biol. 2017 Mar 3;74:15.21.1-15.21.14. doi: 10.1002/cpcb.14. 

A Contra Capture Protein Array Platform for Studying Post-translationally Modified (PTM) Auto-antigenomes. Karthikeyan K, Barker K, Tang Y, Kahn P, Wiktor P, Brunner A, Knabben V, Takulapalli B, Buckner J, Nepom G, LaBaer J, Qiu J. Mol Cell Proteomics. 2016 Jul;15(7):2324-37. doi: 10.1074/mcp.M115.057661. Epub 2016 May 2. 

Advancing translational research with next-generation protein microarrays. Yu X, Petritis B, LaBaer J. Proteomics. 2016 Apr;16(8):1238-50. doi: 10.1002/pmic.201500374. Epub 2016 Mar 31. Review. 

Antiviral antibody profiling by high-density protein arrays. Bian X, Wiktor P, Kahn P, Brunner A, Khela A, Karthikeyan K, Barker K, Yu X, Magee M, Wasserfall CH, Gibson D, Rooney ME, Qiu J, LaBaer J. Proteomics. 2015 Jun;15(12):2136-45. doi: 10.1002/pmic.201400612. Epub 2015 May 15. 

High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays. Yu X, LaBaer J. Nat Protoc. 2015 May;10(5):756-67. doi: 10.1038/nprot.2015.044. Epub 2015 Apr 16. 

NAPPA as a Real New Method for Protein Microarray Generation. Díez P, González-González M, Lourido L, Dégano RM, Ibarrola N, Casado-Vela J, LaBaer J, Fuentes M. Microarrays (Basel). 2015 Apr 24;4(2):214-27. doi: 10.3390/microarrays4020214. Review. 

Microreactor array device. Wiktor P, Brunner A, Kahn P, Qiu J, Magee M, Bian X, Karthikeyan K, LaBaer J. Sci Rep. 2015 Mar 4;5:8736. doi: 10.1038/srep08736. 

Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions. Yu X, Bian X, Throop A, Song L, Moral LD, Park J, Seiler C, Fiacco M, Steel  J, Hunter P, Saul J, Wang J, Qiu J, Pipas JM, LaBaer J. Theranostics. 2014 Jun 6;4(8):808-22. doi: 10.7150/thno.8255. eCollection 2014. 

Robust microarray production of freshly expressed proteins in a human milieu. Festa F, Rollins SM, Vattem K, Hathaway M, Lorenz P, Mendoza EA, Yu X, Qiu J, Kilmer G, Jensen P, Webb B, Ryan ET, LaBaer J. Proteomics Clin Appl. 2013 Jun;7(5-6):372-7. doi: 10.1002/prca.201200063. Epub 2013 May 17. 

A versatile protein microarray platform enabling antibody profiling against denatured proteins. Wang J, Barker K, Steel J, Park J, Saul J, Festa F, Wallstrom G, Yu X, Bian X, Anderson KS, Figueroa JD, LaBaer J, Qiu J. Proteomics Clin Appl. 2013 Jun;7(5-6):378-83. doi: 10.1002/prca.201200062. Epub 2013 May 17. 

Quantifying antibody binding on protein microarrays using microarray nonlinear calibration. Yu X, Wallstrom G, Magee DM, Qiu J, Mendoza DE, Wang J, Bian X, Graves M, LaBaer J. Biotechniques. 2013 May;54(5):257-64. doi: 10.2144/000114028. 

High density diffusion-free nanowell arrays. Takulapalli BR, Qiu J, Magee DM, Kahn P, Brunner A, Barker K, Means S, Miersch S, Bian X, Mendoza A, Festa F, Syal K, Park JG, LaBaer J, Wiktor P. J Proteome Res. 2012 Aug 3;11(8):4382-91. doi: 10.1021/pr300467q. Epub 2012 Jul 13. 

MALDI-TOF characterization of NAPPA-generated proteins. Spera R, Labaer J, Nicolini C. J Mass Spectrom. 2011 Sep;46(9):960-5. doi: 10.1002/jms.1976. No abstract available.  

Nucleic Acid programmable protein arrays: versatile tools for array-based functional protein studies. Miersch S, LaBaer J. Curr Protoc Protein Sci. 2011 Apr;Chapter 27:Unit27.2. doi: 10.1002/0471140864.ps2702s64. 

Nucleic acid programmable protein array a just-in-time multiplexed protein expression and purification platform. Qiu J, LaBaer J. Methods Enzymol2011;500:151-63. doi: 10.1016/B978-0-12-385118-5.00009-8. 

Immunoprofiling using NAPPA protein microarrays. Sibani S, LaBaer J. Methods Mol Biol. 2011;723:149-61. doi: 10.1007/978-1-61779-043-0_10. 

Rapid detection of antibodies in sera using multiplexed self-assembling bead arrays. Wong J, Sibani S, Lokko NN, LaBaer J, Anderson KS. J Immunol Methods. 2009 Oct 31;350(1-2):171-82. doi: 10.1016/j.jim.2009.08.013. Epub 2009 Sep 2. 

Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions: General Guidelines. Link AJ, Labaer J. CSH Protoc. 2008 Dec 1;2008:pdb.ip62. doi: 10.1101/pdb.ip62. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 7: Detecting DNA on NAPPA Slides. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5062. doi: 10.1101/pdb.prot5062. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 6: Detecting Proteins on NAPPA Slides. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5061. doi: 10.1101/pdb.prot5061. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 5: Expressing Proteins on NAPPA Slides. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5060. doi: 10.1101/pdb.prot5060. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 4: DNA Biotinylation, Precipitation, and Arraying of Samples. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5059. doi: 10.1101/pdb.prot5059. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 3: Isolating DNA Plasmids in a 96-Well Plate Format. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5058. doi: 10.1101/pdb.prot5058 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 2: Preparing Bacterial Cultures in a 96-Well Format. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5057. doi: 10.1101/pdb.prot5057. 

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 1: Coating Glass Slides with Amino Silane. Link AJ, Labaer J. CSH Protoc. 2008 Nov 1;2008:pdb.prot5056. doi: 10.1101/pdb.prot5056. 

Tracking humoral responses using self assembling protein microarrays. Ramachandran N, Anderson KS, Raphael JV, Hainsworth E, Sibani S, Montor WR, Pacek M, Wong J, Eljanne M, Sanda MG, Hu Y, Logvinenko T, Labaer J. Proteomics Clin Appl. 2008 Oct;2(10-11):1518-27. doi: 10.1002/prca.200800034. Epub 2008 Sep 10. 

Applications of protein microarrays for biomarker discovery. Ramachandran N, Srivastava S, Labaer J. Proteomics Clin Appl. 2008 Oct;2(10-11):1444-59. doi: 10.1002/prca.200800032. Epub 2008 Sep 10. 

Next-generation high-density self-assembling functional protein arrays. Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG, Rolfs A, Hu Y, LaBaer J. Nat Methods. 2008 Jun;5(6):535-8. doi: 10.1038/nmeth.1210. Epub 2008 May 11. 

Application of protein microarrays for multiplexed detection of antibodies to tumor antigens in breast cancer. Anderson KS, Ramachandran N, Wong J, Raphael JV, Hainsworth E, Demirkan G, Cramer D, Aronzon D, Hodi FS, Harris L, Logvinenko T, LaBaer J. J Proteome Res. 2008 Apr;7(4):1490-9. doi: 10.1021/pr700804c. Epub 2008 Feb 27. 

On-chip protein synthesis for making microarrays. Ramachandran N, Hainsworth E, Demirkan G, LaBaer J. Methods Mol Biol. 2006;328:1-14. 

Emerging tools for real-time label-free detection of interactions on functional protein microarrays. Ramachandran N, Larson DN, Stark PR, Hainsworth E, LaBaer J. FEBS J. 2005 Nov;272(21):5412-25. Review. 

Protein microarrays as tools for functional proteomics. LaBaer J, Ramachandran N. Curr Opin Chem Biol. 2005 Feb;9(1):14-9. Review. 

Self-assembling protein microarrays. Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J. Science. 2004 Jul 2;305(5680):86-90.